ISSN: 2149-2247 | E-ISSN: 2149-2549
Erciyes Medical Journal Exosomes’ Profile in Ankylosing Spondylitis: A Preliminary Study [Erciyes Med J]
Erciyes Med J. 2023; 45(1): 32-40 | DOI: 10.14744/etd.2022.54614

Exosomes’ Profile in Ankylosing Spondylitis: A Preliminary Study

Emel Karakaya1, Rabia Deniz2, Kerem Yiğit Abacar3, Mehmet Pamir Atagündüz3, Can Erzik1
1Department of Medical Biology, Marmara University Faculty of Medicine, İstanbul, Türkiye
2Department of Rheumatology, Başakşehir Çam and Sakura City Hospital, İstanbul, Türkiye
3Division of Rheumatology, Department of Internal Medicine, Marmara University Faculty of Medicine, İstanbul, Türkiye

Objective: Ankylosing spondylitis (AS) is a chronic systemic inflammatory disease that leads to structural and functional impairments and reduced quality of life, with heterogeneous manifestations. The origin and possible role of extracellular vesicles represented by exosomes (EVexo) in the pathogenesis of AS were examined in this study.
Materials and Methods: Extracellular vesicles (EVs) were isolated from serum from ten AS patients and ten healthy controls through Izon qEV2/35 nm columns. After assessing the isolate purity by bicinchoninic acid assay (BCA) and Enzyme-Linked ImmunoSorbent Assay (ELISA), the relationship between EVexo concentration and AS was tested by the BCA method. The EVexo surface markers were analyzed by flow cytometry (FC) to verify EVexo presence and reveal its origin.
Results: In FC analysis, CD86+TSG101+ and CD3+TSG101+ exosome percentages of AS group were significantly higher than the control group (p<0.05). A significant difference was found between the AS and control groups in terms of CD3+IL17+ and CD3+IFNg+ and CD86+TNFα+ and CD86+IL12(p35)+ exosome percentages (p<0.01).
Conclusion: The exosomes whose ratio increased in the AS process were derived from T cells expressing increased levels of IL-17A and IFNg in their membranes, and macrophages expressing increased levels of TNFα and IL-12(p35) in their membranes. The EVexo profile did not change according to the AS course.

Keywords: Exosome, extracellular vesicle, ankylosing spondylitis, cell surface markers.

Emel Karakaya, Rabia Deniz, Kerem Yiğit Abacar, Mehmet Pamir Atagündüz, Can Erzik. Exosomes’ Profile in Ankylosing Spondylitis: A Preliminary Study. Erciyes Med J. 2023; 45(1): 32-40

Corresponding Author: Can Erzik, Türkiye
Manuscript Language: English
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