ISSN: 2149-2247 | E-ISSN: 2149-2549
Erciyes Medical Journal Characterization of Cancer Immune Landscape in Primary Central Nervous System Lymphoma [Erciyes Med J]
Erciyes Med J. 2021; 43(5): 487-493 | DOI: 10.14744/etd.2021.25593

Characterization of Cancer Immune Landscape in Primary Central Nervous System Lymphoma

Fei Fei1, Kai Wang1, Deniz Peker2
1Department of Pathology, University of Alabama at Birmingham, Birmingham, AL, USA
2Department of Pathology, Emory University Faculty of Medicine, Atlanta, GA, USA

Objective: Primary central nervous system lymphoma (PCNSL) is a rare and aggressive extranodal variant of non-Hodgkin lymphomas with disease confined to the brain, spinal cord, leptomeninges, or orbit, without evidence of systemic involvement. PCNSL has a poor prognosis, with a median overall survival of 30–50 months. We used NanoString Technologies to characterize the cancer immune profiles in PCNSL and associated the findings with clinical outcome.
Materials and Methods: A total of 33 patients between 2005 and 2017 were included in our study, and clinical information was collected. A PanCancer IO 360 panel (NanoString Technologies) was used to assess the expression level of 770 genes related to tumor immunity in 12 samples. We also investigated the programmed death ligand 1 (PD-L1) expression and T-cell infiltrate in 25 PCNSL samples using immunohistochemistry.
Results: NanoString analysis showed that gene expressions differed between human immunodeficiency virus (HIV)-positive and HIV-negative PCNSL. We identified four genes with deregulated expressions (adjusted p<0.05) with a cluster enrichment in cytotoxicity, DNA damage repair, interferon signaling, and lymphoid compartment-related genes. Furthermore, we found that PD-L1 was expressed in 9 of 25 (36%) cases and 11 had increased CD3+ T-cell infiltrates. However, increased PD-L1 expression and CD3+ T-cell infiltrates were not correlated with the clinical outcome.
Conclusion: Our data reveals a distinct PCNSL tumor microenvironment and immune landscape. However, our study needs to be validated in a larger population.

Keywords: Primary central nervous system lymphoma, PD-L1, tumor microenvironment.

Fei Fei, Kai Wang, Deniz Peker. Characterization of Cancer Immune Landscape in Primary Central Nervous System Lymphoma. Erciyes Med J. 2021; 43(5): 487-493

Corresponding Author: Deniz Peker, United States of America
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