ISSN: 2149-2247 | E-ISSN: 2149-2549
Erciyes Medical Journal Investigation of Angiotensinogen M235T and T174M Gene Polymorphisms in Coronary Artery Disease [Erciyes Med J]
Erciyes Med J. 2021; 43(5): 475-481 | DOI: 10.14744/etd.2021.08784

Investigation of Angiotensinogen M235T and T174M Gene Polymorphisms in Coronary Artery Disease

Bilge Eren Yamasan1, Tevfik Gülyaşar1, Tammam Sipahi1, Nasır Sivri2, Orkide Palabıyık3
1Department of Biophysics, Trakya University Faculty of Medicine, Edirne, Turkey
2Department of Cardiology, Private Güney Adana Hospital, Adana, Turkey
3Trakya University Health Services and Vocational College, Edirne, Turkey

Objective: Coronary artery disease (CAD) is a multifactorial disorder and is caused by both environmental and genetic factors. As the alterations in angiotensinogen (AGT) gene lead to changes in angiotensin II and plasma levels of AGT, variants of this gene may play a role in CAD pathogenesis. This study aimed to investigate the relationship between CAD and polymorphisms of AGT gene at M235T and T174M regions. Moreover, the associations of potential risk factors with these gene regions and CAD were investigated.
Materials and Methods: In total, the study enrolled 214 cases with CAD and 200 controls. Polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) were used to detect polymorphisms at M235T and T174M. PCR products were electrophoresed on 2% agarose gel, with ethidium bromide, and were then examined under ultraviolet light. Subsequently, RFLP was used to detect gene polymorphisms. A multiple binary logistic regression model was used to investigate the association of risk factors with both CAD and AGT variants.
Results: The number of TT polymorphisms at M235T were significantly higher in the case group than in control group. However, there were no significant differences between cases and controls regarding T174M gene polymorphisms. The presence of hypertension, low high-density lipoprotein level, alcohol consumption, and family history were associated with CAD.
Conclusion: TT polymorphisms at the M235T region in AGT can be an influential factor in the development of CAD.

Keywords: Coronary artery disease, angiotensinogen, M235T, T174M, polymorphism

Bilge Eren Yamasan, Tevfik Gülyaşar, Tammam Sipahi, Nasır Sivri, Orkide Palabıyık. Investigation of Angiotensinogen M235T and T174M Gene Polymorphisms in Coronary Artery Disease. Erciyes Med J. 2021; 43(5): 475-481

Corresponding Author: Bilge Eren Yamasan, Türkiye
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