ISSN: 2149-2247 | E-ISSN: 2149-2549
Volume : 44 Issue : 3 Year : 2022
Leptin Prevents U46619- and Angiotensin II-Elicited Contraction in Isolated Human Umbilical Vessels [Erciyes Med J]
Erciyes Med J. 2022; 44(3): 324-329 | DOI: 10.14744/etd.2021.64249

Leptin Prevents U46619- and Angiotensin II-Elicited Contraction in Isolated Human Umbilical Vessels

İpek Duman, Burak Soner, Salim Yalçın İnan, Ayşe Saide Şahin
Department of Medical Pharmacology, Necmettin Erbakan University, Meram Faculty of Medicine, Konya, Turkey

Objective: This study investigated the vasoactive responses of quiescent and pre-contracted isolated human umbilical veins and arteries to cumulative leptin.
Materials and Methods: The vasoactive response of umbilical vessels pre-contracted with U46619 (10-10M) and angioten-sin II (10-6M) to cumulative leptin (10-11-10-7M) were recorded in vitro.
Results: Leptin did not affect the artery or vein basal tonus (p=1). The leptin-elicited relaxation in U46619-contracted vessels was greater in veins than in arteries (p<0.001). Incubation with N (omega)-nitro-L-arginine methyl ester (L-NAME) (10-4M) prevented the leptin-induced relaxation of the U46619 contraction response in the artery. The Emax and pD2 values of the L-NAME-incubated veins were lower than those of the non-incubated veins (p<0.001 and p=0.001, respectively). The relaxation in angiotensin II-contracted veins was greater than that of the arteries (p<0.001). Incubation with L-NAME prevented leptin-induced relaxation in angiotensin II-contracted arteries. The Emax of leptin-induced relaxation of L-NAME-in-cubated veins was significantly lower than that of non-incubated veins (p=0.027); the pD2 was similar.
Conclusion: Leptin did not alter the resting tension of isolated umbilical vessels; however, the results indicated that leptin caused concentration-dependent relaxation in umbilical vessels pre-contracted with U46619 or angiotensin II. The maximum relaxation was greater in veins compared with arteries. Incubation with L-NAME completely inhibited leptin-induced relax-ation in arteries and resulted in a significant inhibition in veins.

Keywords: Angiotensin II, leptin, U46619, umbilical artery, umbilical vein.

İpek Duman, Burak Soner, Salim Yalçın İnan, Ayşe Saide Şahin. Leptin Prevents U46619- and Angiotensin II-Elicited Contraction in Isolated Human Umbilical Vessels. Erciyes Med J. 2022; 44(3): 324-329

Corresponding Author: İpek Duman, Türkiye
Manuscript Language: English
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