ISSN: 2149-2247 | E-ISSN: 2149-2549
Volume : 44 Issue : 6 Year : 2022
The Effect of Sex-Specific Genetic Factors on the Host Immune Response to COVID-19: A Pilot Study [Erciyes Med J]
Erciyes Med J. 2022; 44(6): 594-602 | DOI: 10.14744/etd.2022.32955

The Effect of Sex-Specific Genetic Factors on the Host Immune Response to COVID-19: A Pilot Study

Dilara Fatma Akın1, Tuğba Arslan Gülen2, Esma Özmen3, Zeynep Türe Yüce4, Orhan Yildiz4, Tuba Turunç5, Uner Kayabaş2
1Department of Medical Biology, Niğde Ömer Halisdemir University Faculty of Medicine, Niğde, Türkiye
2Department of Infectious Diseases and Clinical Microbiology, Niğde Ömer Halisdemir University Faculty of Medicine, Niğde, Türkiye
3Deparment of Medical Biochemistry, Niğde Ömer Halisdemir University Faculty of Medicine, Niğde, Türkiye
4Department of Infectious Diseases and Clinical Microbiology, Erciyes University Faculty of Medicine, Kayseri, Türkiye
5Department of Infectious Diseases and Clinical Microbiology, Health Sciences University Adana Faculty of Medicine, Adana, Türkiye

Objective: The aim of this study was to investigate the impact of sex-specific genetic factors in the pathogenesis and prog-nosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV2)-induced macrophage activation syndrome (MAS), independent of age and comorbidity presence.
Materials and Methods: Patients aged 18-50 years who had been diagnosed with coronavirus 2019 (COVID-19), the disease caused by the SARS-CoV2 virus, were enrolled in a prospective, case-control, multi-center study. Genetic alterations and messenger RNA (m-RNA) expression levels of the TLR7, TLR8, ACE2, CD40L, CXCR3, and TASL genes were determined using DNA sequencing analysis, and gene expression was determined using quantitative reverse transcriptase polymerase chain reaction testing. PolyPhen-2 (Polymorphism Phenotyping v2; Adzhubei et al., 2010) and SNAP2 (Rostlab, Munich, Germany) genetic analysis tools were used to define the pathogenic effects of detected mutations by sequencing the selected genes in hotspot regions.
Results: The study group consisted of 80 patients diagnosed with COVID-19 and was divided into groups based on sex and MAS status. Twenty-nine mutations were detected in 6 genes. Among the alterations, 15 were identified in this study for the first time and 9 were pathogenic. Pathogenic missense mutations in the TLR7, TLR8, ACE2, and TASL genes were detected in the MAS (+) group. In males, decreased TLR7, TLR8, and CXCR3 expression was statistically significant in the MAS (+) group (p<0.050). CXCR3 expression was lower in the female and male MAS (+) groups compared with the MAS (-) groups (p<0.050).
Conclusion: In the absence of major risk factors for COVID-19, the TLR7/8, ACE2, and CXCR3 variants and decreased m-RNA expression levels associated with genetic susceptibility may be independent prognostic risk factors for COVID-19.

Keywords: ACE2, COVID-19, host genetics, mutation, sex difference, TLR7, X chromosome.

Dilara Fatma Akın, Tuğba Arslan Gülen, Esma Özmen, Zeynep Türe Yüce, Orhan Yildiz, Tuba Turunç, Uner Kayabaş. The Effect of Sex-Specific Genetic Factors on the Host Immune Response to COVID-19: A Pilot Study. Erciyes Med J. 2022; 44(6): 594-602

Corresponding Author: Uner Kayabaş, Türkiye
Manuscript Language: English
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