ISSN 2149-2247 | E-ISSN 2149-2549
Original Article
Investigation of Interleukin 1 Alpha Gene Promoter Polymorphism in Hemodialysis Patients with Arteriovenous Fistula Thrombosis
1 Department of Cardiovascular Surgery, Erciyes University Faculty of Medicine, Kayseri, Turkey  
2 Department of Medical Biology, Erciyes University Faculty of Medicine, Kayseri, Turkey  
3 Department of Nephrology, Erciyes University Faculty of Medicine, Kayseri, Turkey  
4 Department of Medical Genetics, Erciyes University Faculty of Medicine, Kayseri, Turkey  
Erciyes Med J 2018; 40: 18-22
DOI: 10.5152/etd.2018.17084
Key Words: Chronic kidney disease, cytokine, interleukin 1 alpha, arteriovenous fistula, thrombosis
Abstract

Objective: Hemodialysis (HD) requires a well-functioning temporary or permanent vascular access. Arteriovenous fistula (AVF) is frequently used in chronic kidney disease (CKD) patients for vascular access. Cytokines direct inflammation and immune response. Inflammation is one of the prevalent risk factors in renal patients. Inflammatory cytokine gene polymorphisms have an effect on vascular access. Therefore, we aimed to investigate a cytokine gene, IL-1α -889 C>T, promoter polymorphism in HD patients with and without AVF thrombosis.

 

Materials and Methods: Eighty-one HD patients and 62 healthy controls were enrolled in the study. Polymorphism was studied using a polymerase chain reaction and restriction fragment length polymorphism. The Mann–Whitney U test and Pearson chi-square analysis were used for statistical analysis.

 

Results: There was no significant difference between the groups. However, the wild genotype was found to be much higher in the group without thrombosis compared to the thrombosis group (59.1% versus 48.6%). This difference was not statistically significant. In the patients’ group, the heterozygous genotype was found to be more prevalent (37.8% versus 27.3%).

 

Conclusions: Polymorphisms in the promoter region of the genes affect the transcriptional activity and also the gene products. According to our results, the IL-1α -889 C>T gene promoter polymorphism is not associated with a risk of thrombosis in HD patients.

 

 

 

Cite this article as: Emiroğulları ÖN, Tunçay A, Şener EF, Taheri S, Ünal A, Özkul Y. Investigation of Interleukin 1 Alpha Gene Promoter Polymorphism in hemodialysis patients with arteriovenous fistula thrombosis. Erciyes Med J 2018; 40(1): 18-22.

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