ISSN 2149-2247 | E-ISSN 2149-2549
Original Article
Anti-apoptotic and antioxidant effect of cerium oxide nanoparticles on cyclophosphamide-induced hepatotoxicity
1 Department of Anatomy, Faculty of Medicine, Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran  
2 Department of Anatomy, Faculty of Medicine, Molecular and Cell Biology Research Center, Mazandaran University of Medical Sciences., Student Research Committee, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran  
3 Department of Toxicology and Pharmacology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran  
4 Department of Radiopharmacy, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran  
Erciyes Med J ; : -
DOI: 10.5152/etd.2018.0016
Key Words: Cyclophosphamide; Nanoceria; Hepatotoxicity; Oxidative Stress; caspase-3.
Abstract

Background: Cyclophosphamide (CP), as an anti-cancer agent has side effects, especially in hepatotoxicity. Cerium oxide nanoparticles (nanoceria; NC), as an antioxidant, have the protective effect against oxidative stress-induced toxicity. This study evaluated the preventive effect of NC in hepatotoxicity induced by CP. Methods: Thirty-two mice were randomly distributed into four groups: Control (received only normal saline), NC (100 μg/kg for 3 days, intraperitoneally), CP (200 mg/kg single dose on the 3th day of study, intraperitoneally) and NC+CP. Two days after the final treatment, oxidative stress markers (MDA and GSH), liver enzymes (ALT, AST, ALP), histopathological and immunohistochemical examinations were performed for determination effects of NC on hepatotoxicity.


Results: CP induced hepatic damage evident by the significant elevation of liver enzymes and alteration in oxidative stress markers. Also, histopathological changes and apoptosis were markedly increased ( P<0.001). NC decreased MDA and increased GSH content and decreased liver enzymes in CP-treated mice (P<0.05 and P<0.01, respectively). In addition, NC pretreatment could be alleviated the immunoreactivity of caspase-3 in CP treated mice compared to the CP group (P<0.001).


In conclusion: NC showed a potent antioxidant in hepatotoxicity mediated by CP. The study suggests that NC through antioxidant and anti-apoptotic properties have the protective effect against hepatotoxicity induced by CP.


Cite this article as: Talebpour Amiri F, Hamzeh M, Yaghubi S, Hosseinimehr SJ. Anti-apoptotic and antioxidant effect of cerium oxide nanoparticles on cyclophosphamide-induced hepatotoxicity. Erciyes Med J 2018; DOI: 10.5152/etd.2018.0016

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